Instrumentation

Proteomics

  • Bruker timsTOF HT Mass SpectrometerNEW in 2023!HT mass spectrometer in laboratory

    • Provides unprecedented depth of proteome coverage and sensitivity via Parallel Accumulation with Serial Fragmentation (PASEF) technology and Trapped Ion Mobility Spectrometry (TIMS)-mediated gas phase separations of ions for increased selectivity prior to mass analysis
    • Features maximum MS and MS/MS scan rates of ~150 Hz
    • Capable of multiple mass spectral acquisition options that incorporate PASEF technology, including data-dependent acquisition (DDA-PASEF), data-independent acquisition (DIA-PASEF), and parallel-reaction monitoring (PRM-PASEF) modes
    • Directly coupled to a Thermo Scientific Dionex Ultimate 3000 RSLCnano UPLC for high resolution, reversed-phase liquid peptide separations at nanoflow rates for maximum sensitivity

 

 

 

 

 

  • Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer

    Orbitrap Eclipse

    • Provides multiple mass analysis and detection modes for MS and MS/MS using an Orbitrap or dual-pressure linear ion trap
    • Features multiple types of gas-phase fragmentation including collision-induced dissociation (CID), higher energy C-trap dissociation (HCD), electron transfer dissociation (ETD) plus hybrid versions of these techniques: EThcD and ETciD
    • Equipped to perform Real-Time Search (RTS) and Synchronous Precursor Selection (SPS) MS3-based acquisition methods for multiplexed proteomics analyses that incorporate Thermo Scientific Tandem Mass Tags (TMT) reagents for peptide quantitation
    • Capable of multiple mass spectral acquisition options including data-dependent acquisition (DDA), data-independent acquisition (DIA), and parallel-reaction monitoring (PRM) modes
    • Directly coupled to a Thermo Scientific Dionex Ultimate 3000 RSLCnano UPLC for high resolution reversed-phase liquid peptide/protein separations at variable flow regimes, including nanoflow rates for maximum sensitivity

 

 

 

 

 

 

  • Q Exactive HFThermo Scientific Q Exactive HF Mass Spectrometer

    • Provides high resolution, accurate mass determinations for MS and MS/MS analysis of proteins and peptides in complex mixtures using High-Field (HF) Orbitrap technology
    • Capable of multiple mass spectral acquisition options including data-dependent acquisition (DDA), data-independent acquisition (DIA), and parallel-reaction monitoring (PRM) modes
    • Directly coupled to a Thermo Scientific Vanquish Neo UPLC for high resolution reversed-phase liquid peptide/protein separations at variable flow regimes, including nanoflow rates for maximum sensitivity

       

       

       

       

       

       

       

      • Available software for proteomics analysis: MaxQuant (Ref), Spectronaut v17 (Biognosys)NEW in 2023!, Proteome Discoverer v2.5 with XlinkX node (Thermo Scientific), Byonic (Protein Metrics, Inc.), Scaffold v5.0 (Proteome Software, Inc.)

       

      Metabolomics

      • Bruker timsTOF HT Mass SpectrometerNEW in 2023!HT mass spectrometer in laboratory

        • Provides unprecedented depth of metabolome coverage and sensitivity via Parallel Accumulation with Serial Fragmentation (PASEF) technology and Trapped Ion Mobility Spectrometry (TIMS)-mediated gas phase separations of ions for increased selectivity prior to mass analysis
        • Features maximum MS and MS/MS scan rates of ~150 Hz
        • Capable of multiple mass spectral acquisition options that incorporate PASEF technology, including data-dependent acquisition (DDA-PASEF), data-independent acquisition (DIA-PASEF), and parallel-reaction monitoring (PRM-PASEF) modes
        • Directly coupled to a Thermo Scientific Vanquish Flex UPLC for high resolution, multi-mode liquid metabolite separations at micro-flow rates

       

       

       

       

       

      • Waters Synapt G2-Si q-TOF Mass Spectrometer

        Synapt G2Si HDMS

        • Provides high resolution, accurate mass measurements for MS and MS/MS analysis of small molecules in complex mixtures using q-TOF technology
        • Capable of multiple mass spectral acquisition options including DDA and DIA modes (MSe)
        • Ion mobility technology provides another dimension of separation based on collisional-cross section (analyte size and shape) when mass and retention time aren’t sufficient to separate chemically ambiguous analytes and/or isomers
        • Directly coupled to a Waters Acquity UPLC for high resolution liquid-phase separations at micro-flow rates (μL/min)

         

         

         

         

         

         

         

         

        • Xevo G2-XSWaters Xevo G2-XS q-TOF Mass Spectrometer – with SONAR

          • Provides high resolution, accurate mass measurements for MS and MS/MS analysis of small molecules in complex mixtures using q-TOF technologyCapable of multiple mass spectral acquisition options including DDA and DIA modes (MSe and SONAR)
          • Directly coupled to a Waters H-Class UPLC with Quaternary Solvent Manager for complex liquid-phase separations at micro-flow rates (μL/min) and binary- to quaternary-style gradient modes

           

           

           

           

           

           

           

           

          • Available software for large-scale, untargeted metabolomics analysis: Metaboscape (Bruker)NEW in 2023!, Progenesis QI (Nonlinear Dynamics)